|Location : Greifswald / D|
|Leader : Michael Hecker|
|Website : www.uni-greifswald.de|
- Description of the institution:
The Institute of Microbiology in Greifswald is an international reference laboratory for the proteomics of Gram-positive bacteria. A comprehensive model of cell physiology relying on proteomics and transcriptomics has been already established for the bacterium Bacillus subtilis. The Institute is responsible for the unique Bacillus subtilis protein database “Sub-2D” ( http://microbio2.biologie.uni-greifswald.de:8880/sub2d.htm ). The 2D protein gel electrophoresis technique, including N-terminal sequencing of proteins, high throughput protein identification using MALDI-TOF- and MALDI-TOF-TOF-MS, or computer-aided evaluation of 2D gels (including matching, dual channel imaging, DIGE technology etc.) is well established. Additionally the techniques for comparative protein expression profiling relying on gelfree approaches (MDLC-ESI-MS/MS, MDLC-MALDI-MS/MS) are established and applied ruinously. The institute also has a wide experience in the characterization of stress-inducible chaperones/proteases and in techniques of molecular microbiology.
- Key persons involved in BaSysBio:
Prof. Michael Hecker
Head of the Institute of Microbiology at EMAU. The major research topics of his group are: (i) Analysis of global regulation of gene expression in B. subtilis using the proteome approach as well as transcriptional techniques (incl. DNA microarrays); (ii) Physiology and molecular biology of specific and general stress/starvation proteins in B. subtilis ; (iii) Proteomics of B: subtilis ; (iv) Stress response and expression of virulence genes in Gram-positive pathogenic bacteria ( Staphylococcus aureus ).
Dr. Dörte Becher
Dr. Becher is in charge of the mass spectrometry group in the Institute of Microbiology. She has experiences in mass spectrometry for more then 10 years. The analysis of proteins, their quantitation and the characterization of modifications is in focus of her work. She will be responsible for the adaptation of the gel-free proteome analysis relying on MDLC-MS/MS and the analysis of TF based on mass spectrometry.
Dr. Ulrike Mäder
Dr. Mäder is in charge of the microbial transcriptomics in Greifswald. She has many years experience in the field of DNA array and proteome technologies. Dr Mäders work is focused on the regulatory networks of cellular adaptation to stress and starvation in Gram-positive bacteria. She will be responsible for microarray analyses of Bacillus subtilis stress response in particular with regard to mRNA processing and stability.